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Voltage‐dependent potassium currents in cultured trout oligodendrocytes
Author(s) -
Glassmeier G.,
Jeserich G.,
Krüppel T.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490320302
Subject(s) - tetraethylammonium , membrane potential , biophysics , trout , potassium , potassium channel , reversal potential , chemistry , patch clamp , resting potential , conductance , voltage gated potassium channel , electrophysiology , neuroscience , biology , physics , organic chemistry , condensed matter physics , fishery , fish <actinopterygii>
Ionic currents were recorded in cultured oligodendrocytes from the brain of trout using the whole‐cell configuration of the patch‐clamp technique. Outward currents were evoked at membrane potentials more positive than −40 mV, which could be separated into two components according to their kinetic parameters and their sensitivity to the holding potential: a fast inactivating current which was completely suppressed by 4‐aminopyridine and reduced by tetraethylammonium and a slow steady‐state conductance which was similarly sensitive to both potassium channel blockers. The current reversal potential was close to the potassium equilibrium potential. In contrast to mammalian oligodendrocytes but in similarity with cultured Schwann cells, trout oligodendrocytes did not exhibit any inwardly rectifying currents at hyperpolarized membrane potentials. © 1992 Wiley‐Liss, Inc.