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Analysis of promoter activity and 5′ genomic structure of the neural cell adhesion molecule L1
Author(s) -
Kohl A.,
Giese K. P.,
Mohajeri M. H.,
Montag D.,
Moos M.,
Schachner M.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490320206
Subject(s) - exon , biology , intron , exon trapping , microbiology and biotechnology , coding region , gene , tata box , exon shuffling , tandem exon duplication , genetics , consensus sequence , promoter , alternative splicing , gene expression , peptide sequence
To gain insight into the molecular mechanisms underlying the regulation of expression of the neural cell adhesion molecule L1 and into the exon–intron structure of the L1 gene, a genomic clone from the mouse was characterized. The clone was identified by screening an EMBL3 library with an L1‐specific cDNA probe and comprises approximately 15 kb, in which the first 2,206 nucleotides of the coding region are included. Of the 5 of 6 immunoglobulin (Ig)‐like domains sequenced, all are encoded by 2 exons, with the first exon being smaller than the second. The exon encoding the signal peptide is separated from a mini‐exon containing 15 bp by a large intron, approximately 2.6 kb in length, whereas the other introns are smaller, with the coding information for the Ig‐like domains 3–5 clustered in a 1,643‐bp‐long fragment with introns only 110–217 bp in length. The 5′ upstream region of the clone comprises 5 kb, with the first 112 bp lying upstream to the coding sequence and containing a start site for transcription. No consensus sequence for a TATA box was found. Consensus DNA sequences for the binding of the gene products of Hox 1.3, engrailed and bicoid, are localized upstream to the transcription start site. A 1,262‐bp fragment containing part of the first exon showed promoter activity in neuroblastoma cells, but hardly in L cells and not in CHO cells, indicating that this fragment is sufficient for neural cell directed promoter activity. © 1992 Wiley‐Liss, Inc.