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An immortalized cell line expresses properties of activated microglial cells
Author(s) -
Bocchini V.,
Mazzolla R.,
Barluzzi R.,
Blasi E.,
Sick P.,
Kettenmann H.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490310405
Subject(s) - immortalised cell line , cell culture , microbiology and biotechnology , in vitro , microglia , recombinant dna , biology , chemistry , immunology , inflammation , biochemistry , gene , genetics
Murine cultured microglial cells were immortalized after infection with a v‐raf/v‐myc recombinant retro‐virus. This immortalized cell line (BV‐2) shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. BV‐2 cells are not constitu‐tively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in [Ca ++ ] i . BV‐2 cells, like microglial cells, are however, distinct from peripheral macrophages by their expression of inwardly rectifying K + cannels in concert with a Jack in outwardly rectifying K + channels and the formation of spineous processes. The BV‐2 cell line thus represents a suitable model for in vitro studies of activated microglial cells.