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Transient, cyclic changes in mouse visual cell gene products during the light‐dark cycle
Author(s) -
McGinnis J. F.,
Whelan J. P.,
Donoso L. A.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490310325
Subject(s) - visual phototransduction , rhodopsin , arrestin , biology , messenger rna , microbiology and biotechnology , gene , gene expression , biophysics , retina , genetics , biochemistry , neuroscience , signal transduction , retinal , g protein coupled receptor
Temporal and spatial changes in the cellular and sub‐cellular concentrations of photoreceptor cell gene products appear to be important features of pho‐totransduction in rod photoreceptor cells. The time course of the rapid, light‐dependent movement of S‐antigen (S‐Ag) (48k; Arrestin) from the inner segments to the outer segments was analyzed using poly‐clonal and monoclonal antibodies. The concentrations of mRNA change about threefold for rhodopsin and about sixfold for S‐Ag in cyclic, independent modes during the normal light‐dark cycle. Kinetic analysis indicates that the oscillations of S‐Ag mRNA levels are due to changes in the transcriptional activity of the gene itself. An experimental model is presented summarizing the relationships between mRNA levels, protein localization, disc shedding, and phototransduction in the photoreceptors cells.