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Cytoskeletal elements regulate the distribution of nerve growth factor receptors in PC12 cells
Author(s) -
Spoerri P. E.,
Roisen F. J.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490310312
Subject(s) - cytochalasin b , cytoskeleton , microtubule , receptor , endoplasmic reticulum , microbiology and biotechnology , microfilament , immunogold labelling , nerve growth factor , biology , cytochalasin d , chemistry , antibody , cell , biochemistry , immunology
Abstract Nerve growth factor receptor (NGFR)‐like immunoreactivity (IR) was studied in PC12 cells treated for 96 hr with NGF (40 ng/ml), using immunogold labeling and electron microscopic morphomctric analysis. The cells were exposed to the anti‐NGFR antibody 192‐IgG, followed by immunoglobulin (IgG) conjugated with colloidal gold. PC12 cells exhibited occasional gold label (positive NGFR‐IR) on all surfaces. Cells treated with colcemid (0.05 μg/ml) or cytochalasin D (2 μg/ml), which limit microtubule (MT) and microfilament (MF) formation, respectively, displayed an increased NGFR‐IR in terms of gold labeling. NGFR‐IR was also seen on taxol (0.85 μg/ml)‐exposed cells, an agent that promotes MT assembly. Cells treated simultaneously with cytochalasin D and taxol had a dramatically augmented NGFR‐IR on their surfaces, which exceeded levels obtained with either agent alone. Prominent NGFR‐IR was localized frequently in coated endocytotic vesicles, in smooth endoplasmic reticulum, and in secondary multivesicular lysosomes, in both treated and untreated cells. The results suggest that a large number of NGFRs (positive NGFR‐IR) in PC12 cells are cryptic and not available for ligand binding. Changes in cytoskeletal organization that may affect mobility of integral membrane proteins can modulate the distribution of NGFR‐IR on neuronal surfaces.