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Selective susceptibility of cultured striatal neurons to kainic acid
Author(s) -
Mesco E. R.,
Joseph J. A.,
Roth G. S.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490310216
Subject(s) - kainic acid , receptor , spiperone , biology , microbiology and biotechnology , ligand (biochemistry) , in vitro , cell culture , neuron , medicine , neuroscience , endocrinology , dopamine receptor , biochemistry , genetics , glutamate receptor
Previous work has shown a selective sensitivity of striatal D 2 ‐receptor‐containing neurons (D 2 cells) to kainic acid (KA). In order to see whether this phenomenon exists in cultured cells, which could provide a very accessible model for subsequent mechanistic studies, we examined striatal cultures grown for up to 30 days and labeled with either [ 3 H] spiperone, for D 2 ‐receptors, or with [ 3 H] ‐SCH23390, for D1 receptors. Analysis of the cells was performed with a digital imaging system (RAS). The cultured cells were examined for ligand binding to receptors, cell size, and susceptibility to KA. The D 2 ‐cells showed an increased mortality over D 1 ‐receptor‐containing neurons (D 1 cells) in the presence of KA, of a magnitude similar to in vivo loss, with larger D 2 ‐cells showing the greatest vulnerability.