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Substance P immunoreactivity of rat brain stem neurons in primary culture
Author(s) -
Jacquin T. D.,
Denizot J. P.,
DenavitSaubie M.
Publication year - 1992
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490310118
Subject(s) - immunocytochemistry , biotinylation , neurotransmitter , in vitro , embryonic stem cell , primary culture , substance p , microbiology and biotechnology , biology , phosphatase , chemistry , neuroscience , endocrinology , biochemistry , central nervous system , receptor , gene , neuropeptide , phosphorylation
Substance P (SP)‐ergic neurons from 16/17 day‐embryonic rat brain stem in primary culture were identified by immunocytochemistry using biotinylated avidine and phosphatase alcaline methods with affinity‐purified anti‐SP antibodies. An average of 84% of neurons contained SP from day 9 to day 21 after plating. These in vitro data show that SP‐containing neurons develop in our culture conditions. SP may act as a maturation factor as well as a neurotransmitter.