Premium
Protein kinase C stimulation enhances the process formation of adult oligodendrocytes and induces proliferation
Author(s) -
Althaus H. H.,
Schröter J.,
Spoerri P.,
Schwartz P.,
Klöppner S.,
Rohmann A.,
Neuhoff V.
Publication year - 1991
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490290408
Subject(s) - stimulation , protein kinase c , microbiology and biotechnology , in vitro , kinase , chemistry , protein kinase a , biochemistry , biology , endocrinology
Oligodendrocytes (OL) isolated from adult pig brains regenerate their processes and form a network of fibers after 14–18 days in vitro (DIV). Stimulation of protein kinase C (Pk‐C) by tumour promoters such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) produces at day 7 in vitro a similar network after only 20 hr. H‐7, an inhibitor of Pk‐C, as well as amiloride, which inhibits the subsequent Na + /H + exchange, reversibly suppress this effect. Activation of the protein kinase A or calmodulin pathway do not result in an increased OL process production. Furthermore, TPA induced proliferation in a subpopulation of OL. We conclude that the stimulation of Pk‐C is of utmost importance for OL regeneration.