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Quantitation of the growth‐associated protein B‐50/GAP‐43 and neurite outgrowth in PC12 cells
Author(s) -
San E. R. A. Jap Tjoen,
SchmidtMichels M. H.,
Spruijt B. M.,
Oestreicher A. B.,
Schotman P.,
Gispen W. H.
Publication year - 1991
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490290203
Subject(s) - neurite , nerve growth factor , intracellular , microbiology and biotechnology , chemistry , in vitro , biology , biochemistry , receptor
A combined assay to measure neurite outgrowth and B‐50/GAP‐43 levels in PC 12 cells is reported. During NGF‐induced neuritogenesis, B‐50/GAP‐43 expression was monitored by enzyme‐linked immunosorbent assay (ELISA). Neurite outgrowth was quantified at the same time by the use of video image analysis. Sensitivity and reliability of the methods are shown with a dose–response and time curve of β‐NGF‐induced neuritogenesis. A linear increase in total length of neurites was induced by concentrations of β‐NGF ≥5 ng/ml and was accompanied by a linear increase in the amount of B‐50/GAP‐43. The combined methods presented here can conveniently and reliably establish subtle changes in neurite outgrowth and intracellular protein contents.