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Inhibition of mouse T‐cell proliferation by CGRP and VIP: Effects of these neuropeptides on IL‐2 production and cAMP synthesis
Author(s) -
Boudard F.,
Bastide M.
Publication year - 1991
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490290104
Subject(s) - calcitonin gene related peptide , neuropeptide , microbiology and biotechnology , chemistry , cell growth , endocrinology , medicine , biology , biochemistry , receptor
We compared the effect of two neuropeptides, calcitonin generelated peptide (CGRP) and vasoactive intestinal peptide (VIP), on mitogen‐induced murine splenocyte proliferation. Both neuropeptides exerted their maximal effect within 24 hr after activation by Con A. The combination CGRP‐VIP caused an additive inhibitory effect on T‐cell proliferation. The inhibitory effect of VIP could be correlated with a decrease in interleukin 2 (IL‐2) production, whereas CGRP did not affect this production. Since we also observed an additive inhibitory effect on T‐cell proliferation by the theophylline and CGRP or VIP combination, we measured the effect of each neuropepntide on intracellular cAMP production by enriched T‐cells: CGRP, but not VIP, strongly stimulated cAMP synthesis. Taken together, our results indicate that inhibition of murine T‐cell proliferation by CGRP and VIP is mediated by different mechanisms.