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Expression and induction of intercellular adhesion molecules (ICAMs) and major histocompatibility complex (MHC) antigens on cultured murine oligodendrocytes and astrocytes
Author(s) -
Satoh J.,
Kim S. U.,
Kastrukoff L. F.,
Takei F.
Publication year - 1991
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490290102
Subject(s) - major histocompatibility complex , microbiology and biotechnology , antigen , biology , astrocyte , adhesion , cell adhesion molecule , immunology , chemistry , neuroscience , central nervous system , organic chemistry
Expression of intercellular adhesion molecule‐1 (ICAM‐1), ICAM‐2‐like molecule (Lgp55), and class I/II major histocompatibility complex (MHC) antigens (H‐2 and Ia) was investigated in cultures of mu‐rine Oligodendrocytes and astrocytes. Under unstim‐ulated conditions, low levels of ICAM‐1 expression were observed on astrocytes (<20%), but not on oligodendrocytes. Lgp55 was expressed intensely on oligodendrocytes (>90%) and to a lesser degree on astrocytes (>70%). A weak class I MHC (H‐2) im‐munoreactivity was identified on both Oligodendrocytes and astrocytes (50‐70%). Class II MHC (Ia) antigen was undetectable on both cell types. After 48‐hr exposure to immune mediators that include interferon‐γ (IFN‐γ), 500 U/ml, and supernatant from concanavalin A (Con A)‐activated spleen cells, ICAM‐1 expression was markedly increased on astrocytes (>80%), but not on Oligodendrocytes. Lgp55 expression on both cell types was not altered. Induction of H‐2 antigen expression by immune mediators was quite high on both cell types (>95%), while Ia antigen induction was low on astrocytes (<50%) and did not occur on Oligodendrocytes. Cell type‐specific expression and induction of ICAMs and MHC antigens by immune mediators may play roles in lymphocyte‐glial cell interactions at the sites of inflammation in the central nervous system (CNS).