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Regulation of heat shock protein synthesis in rat astroeytes
Author(s) -
Dwyer B. E.,
Nishimura R. N.,
De Vellis J.,
Clegg K. B.
Publication year - 1991
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490280306
Subject(s) - heat shock protein , shock (circulatory) , protein biosynthesis , messenger rna , heat shock , microbiology and biotechnology , hsp70 , methionine , biology , biochemistry , gene , medicine , amino acid
Rat forebrain astrocytes synthesize heat shock proteins with molecular weights 97,89,70,68, and 30‐34 kilodaltons. The stress inducible 68‐kDa heat shock protein (HSP‐68) was vigorously expressed by astro‐cytes in culture after a 45°C, 20 min heat shock. HSP‐68 synthesis was poorly inducible by a second heat shock given 16 hr after the initial heat shock. Decreased [ 35 S]methionine incorporation into HSP‐68 correlated with low levels of HSP‐68 mRNA present after the second heat shock. The data suggest that control of HSP‐68 mRNA levels by transcriptional/ posttranscriptional mechanisms is a major site for regulation of HSP‐68 synthesis.