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Nerve growth factor‐induced increase in calcium uptake by PC12 cells
Author(s) -
Nikodijevic B.,
Guroff G.
Publication year - 1991
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490280206
Subject(s) - nerve growth factor , calcium , endocrinology , chemistry , medicine , pharmacology , biology , receptor
Treatment of PC 12 cells with nerve growth factor (NGF) produces a rapid and transient increase in calcium uptake into the cells. The increased uptake is maximal after 5 minutes of NGF treatment, but after 15 minutes of NGF treatment, no such increase can be observed. The effect of NGF is partially inhibited by blockers of L‐type calcium channels. K‐252a, an alkaloid‐like kinase inhibitor that usually is found to inhibit the actions of NGF on PC 12 cells, produces an increase in calcium uptake similar to, but smaller than, that seen with NGF. NGF had no effect on calcium release under these conditions.