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Regulation of β‐nerve growth factor expression by inflammatory mediators in hippocampal cultures
Author(s) -
Friedman W. J.,
Lärkfors L.,
AyerLeLievre C.,
Ebendal T.,
Olson L.,
Persson H.
Publication year - 1990
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490270316
Subject(s) - nerve growth factor , hippocampal formation , endocrinology , medicine , activator (genetics) , lipopolysaccharide , biology , messenger rna , in vitro , chemistry , microbiology and biotechnology , biochemistry , receptor , gene
Substances which regulate expression of nerve growth factor (NGF) were examined in embryonic rat: hippocampal cultures containing both neurons and glial cells. Both cell types expressed NGF mRNA when cultivated in vitro. Lipopolysaccharide, an activator of macrophages, elicited a significant increase in NGF mRNA. Interleukin‐1β evoked a similar increase in NGF mRNA which was accompanied by a rise in NGF protein. The II‐1‐induced increase was partially blocked by indomethacin, suggesting that prostaglandins might mediate this effect. Treatment of the cultures directly with prostaglandin E 2 resulted in elevated levels of both NGF mRNA and protein. Thus, agents which promote inflammatory activity appear to increase NGF expression. Moreover, a suppressor of inflammation, dexamethasone, decreased NGF expression. Our observations indicate that a variety of immunomodulators regulate NGF expression in the hippocampus.