Premium
Changes in PC12 cell morphology induced by transfection with 42C cDNA, coding for a member of the S‐100 protein family
Author(s) -
Masiakowski P.,
Shooter E. M.
Publication year - 1990
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490270304
Subject(s) - complementary dna , transfection , nerve growth factor , messenger rna , biology , coding region , dna , rna , family member , microbiology and biotechnology , cell culture , gene , genetics , receptor , medicine , family medicine
The cloned DNA coding for 42C protein (light chain of calpactin I), whose mRNA is induced in PC12 cells by treatment with nerve growth factor (NGF), was reintroduced into these cells. A cell line was obtained in which the outgrowth of processes in the absence of added NGF, similar to that induced in the parental PC12 cells by the factor, was accompanied by high levels of 42C RNA. The apparent reason for this constitutive overexpression of 42C is the stable integration of multiple copies of the 42C DNA into the cell genome. These results further support the notion that S‐100 protein family, of which 42C is a member, may play an important role in development.