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Patterns of cerebral cortex mRNA expression
Author(s) -
Bernal J.,
Godbout M.,
Hasel K. W.,
Travis G. H.,
Sutcliffe J. G.
Publication year - 1990
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490270205
Subject(s) - cerebrum , cerebral cortex , biology , cortex (anatomy) , suppression subtractive hybridization , cerebellum , cdna library , northern blot , in situ hybridization , microbiology and biotechnology , complementary dna , gene expression , temporal cortex , rna , central nervous system , neuroscience , gene , genetics
A pool of 163 clones, isolated by screening 60,000 members of a Macaca fascicularis cerebral cortex cDNA library with a cortex‐minus‐cerebellum subtracted probe prepared by the phenol enhancement method, was analyzed by Northern blot hybridization studies. One hundred fifty‐three of these clones corresponded to 22 RNAs whose abundance was at least 2‐fold higher in cerebral cortex poly (A) + RNA samples than in samples of cerebellar poly(A) + Seven of these RNAs, represented by 131 clones, were undetectable in cerebellum. Only 10 of the 163 clones proved to be false positives. The abundance of several of these cortex‐enriched RNAs was altered in Alzheimer's disease brains. Several RNAs that were present in cerebral cortex but undetectable in cerebellum were generally enriched in telencephalon, although none was restricted to the cortex. One of the cortex enriched RNAs, whose nucleotide sequence is presented, encoded monkey preprocholecystokinin. Overall, this study provides insights into the powers and limitations of subtractive hybridization and into the patterns of gene expression in the central nervous system.