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Morphological changes in cultures of hippocampus following prenatal irradiation in the rat
Author(s) -
Hamdorf G.,
Shahar A.,
CervósNavarro J.,
Schemer A.,
Sparenberg A.,
Skoberla A.
Publication year - 1990
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490260309
Subject(s) - hippocampus , synaptogenesis , myelin , golgi apparatus , biology , irradiation , fetus , chemistry , pathology , microbiology and biotechnology , central nervous system , endocrinology , pregnancy , medicine , physics , nuclear physics , genetics , endoplasmic reticulum
The effect of prenatal irradiation was studied in organotypic cultures of hippocampus, prepared from newborn rats that had been exposed to wholebody irradiation of 1 Gy from a 60 Co‐source at day 13 of pregnancy. Light and electron microscopic observaions showed remarkable damage to neuronal mitochondria accompanied by extensive swelling, vacuolation of the Golgi complex, and formation of multilamellar bodies and vesicles of the lysosomal type. In contrast to neuronal alterations, no delay in synaptogenesis or onset of myelination was observed based upon the absence of significant morphological changes in synapses and myelin sheaths. Using this tissue culture model it could be confirmed that prenatal exposure to irradiation, even at low doses, induces specific morphological changes in the brain.