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Tyrosine hydroxylase gene expression in human ventral mesencephalon: Detection of tyrosine hydroxylase messenger RNA in neurites
Author(s) -
Dumas S.,
JavoyAgid F.,
Hirsch E.,
Agid Y.,
Mallet J.
Publication year - 1990
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490250415
Subject(s) - tyrosine hydroxylase , substantia nigra , neurite , midbrain , messenger rna , complementary dna , polyclonal antibodies , biology , microbiology and biotechnology , tyrosine 3 monooxygenase , in situ hybridization , immunocytochemistry , tyrosine , rna , gene expression , cell bodies , gene , central nervous system , endocrinology , antibody , dopamine , biochemistry , immunology , dopaminergic , in vitro
The distribution of both tyrosine hydroxylase (TH) antigen and messenger RNA was investigated in the ventral mesencephalon from human brain using a 35 S‐labeled human TH cDNA probe and a polyclonal TH antibody. A hybridization signal was detected not only in the perikaryon but also in the neurites, most probably dendrites, of immunoreactive neurons. In cell bodies, the level of TH mRNA varies, revealing a regional heterogeneity. In the substantia nigra of patients with Parkinson's disease the number of labeled cells was markedly reduced as compared to controls.