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Enzymatic activites during differentiation of the human neuroblastoma cells, LA‐N‐1 and LA‐N‐2
Author(s) -
Singh I. N.,
Sorrentino G.,
McCartney D. G.,
Massarelli R.,
Kanfer J. N.
Publication year - 1990
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490250405
Subject(s) - cell culture , enzyme assay , endocrinology , enzyme , choline acetyltransferase , acetylcholinesterase , in vitro , microbiology and biotechnology , medicine , choline , biology , tyrosine hydroxylase , chemistry , biochemistry , cholinergic , genetics
The presence of 10 −5 M retinoec acid (RA) in the culture medium of LA‐N‐1, a catecholamonergic cell line, and LA‐N‐2, a cholinergic cell line, enhanced their morphological differentiation. Tyrosine hydroxylase (TH) activity of the LA‐N‐1 cell was increased in the RA‐treated cells compared with control cultures at day 4 and remained elevated. Choline actyltransferase (ChAT) activity in the LA‐N‐2 cells gradually increased until 8 days in vitro (DIV) voth in the untreated control and the RA treated cultures. This activity in control and treated cells decreased gradually to a constant level of activity. The ChAT activity at 8 DIV of RA‐treated La‐N‐2 cells was increased 2.1‐fold (P<0.001) as compared to the control cultures. This increase in ChAT activity was accompanied by a 73% decrease of acetylcholinesterase (AChE) activity in LA‐N‐2 cell by 8DIV. AChE activity of LA‐N‐1 cells was unchanged during the time course of the experiment. Phospholipase‐A 2 (PL‐A 2 )activity in RA‐treated LA‐N‐2cells was increased at day 4 as compared with the control cultures. There were no differnces obseved in phopholipase‐D (PL‐D), choline kinase and FPC‐phosphodiesterases activities in RA‐treated and ‐untreated LA‐N‐1 and LA‐N‐2 cells.