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Neuron‐specific splicing of C‐SRC cDNA RNA in human brain
Author(s) -
Pyper J. M.,
Bolen J. B.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490240113
Subject(s) - exon , fyn , biology , alternative splicing , intron , complementary dna , gene , rna splicing , coding region , homology (biology) , proto oncogene tyrosine protein kinase src , microbiology and biotechnology , rna , genetics , kinase
The C ‐ SRC , C ‐ YES , and FYN genes encode three closely related tyrosine protein kinases that are expressed in human neural tissues. A unique form of the C ‐ SRC gene has been demonstrated to be expressed in avian and murine brain tissues as the result of alternative splicing between the third and fourth exons. This neuronal‐specific splicing event adds to the C ‐ SRC mRNA an 18 base pair exon capable of encoding the same six amino acids in both avian and murine neural tissues. The C – YES and FYN genes share with C ‐ SRC similar exon‐intron boundaries and a high degree of amino acid sequence homology in the 3/4 exon coding region. However, potential alternative splicing of the C ‐ YES and FYN genes in this region has not been previously investigated. In this study we have compared the expression of C ‐ SRC , C ‐ YES , and FYN RNAs in human lung, liver, brain, and placenta tissues and prepared cDNA clones spanning exons 3 and 4 for each of these genes from the different tissues. Sequence analysis of these cDNA clones revealed that the splicing patterns for the FYN and C ‐ YES genes were the same among the various tissues, whereas C ‐ SRC cDNAs isolated from brain contained 18 additional bases with the capacity to code for the same six amino acids present in the neural‐specific forms of avian and murine pp60 c‐src .