Premium
Evidence from neuronal heterokaryons for a trans‐acting factor suppressing THY‐1 Expression during neuronal development
Author(s) -
Saleh M.,
Bartlett P. F.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490230406
Subject(s) - in vitro , heterokaryon , microbiology and biotechnology , immunofluorescence , trans acting , biology , suppressor , chemistry , gene , immunology , genetics , antibody , mutant
Abstract The expression of Thy‐1 on developing sensory neurons cultured in vitro is strictly regulated and follows a temporal course identical to that observed in situ. We have investigated the role of trans‐acting factors in this innate regulation by constructing heterokaryons between mature Thy‐1.1 + expressing neurons and immature Thy‐1.2 − neurons. It has been observed by immunofluorescence that within 16 hr of fusion, Thy‐1.1 expression is suppressed in such heterokaryons. However, this suppression is reversible, and after 3–4 days in vitro, at the time at which Thy‐1.2 is normally expressed, there is re‐expression of the Thy‐1.1 molecule producing Thy‐1.1 + /Thy‐1.2 + co‐expressing heterokaryons. As nuclear fusion does not occur, it appears that a developmentally regulated diffusible suppressor molecule is responsible for these observations.