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Immunohistochemistry with anti‐calbindin and anti‐neurofilament antibodies in the cerebellum of methylazoxymethanol‐treated mice
Author(s) -
de Barry J.,
Gombos G.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490230313
Subject(s) - immunohistochemistry , calbindin , neurofilament , cerebellum , antibody , neuroscience , biology , microbiology and biotechnology , pathology , immunology , medicine
Mice pups were injected with methylazoxymethanol at birth (MAM 0 ) or on the fifth postnatal day (MAM 5 ) and their cerebella were examined when adult. Immunohistochemistry with an antiserum directed against calbindin, a protein specific for Purkinje cells, was used to survey more easily Purkinje cell position and orientation. For a general view of basket cell axon distribution, we used a monoclonal antibody that recognized the phosphorylated form of the 200 kD constituent protein of neurofilaments, which is axon specific. The present results confirm that in MAM 5 the cytoarchitecture was preserved, some Purkinje cells degenerated, and the pericellular basket around the Purkinje cells was apparently normal. In MAM 0 animals, the Purkinje cells appeared malpositioned and disoriented, the pinceau around the Purkinje cell hillock was absent, but basket cell axons were present. This indicated that the absence of pinceau was not due to the absence of basket cells, but probably to alterations of cell interactions, which hindered the proper pericellular basket formation.