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Quantitative analysis of the localization of adrenergic binding sites in chick brain
Author(s) -
Dermon C. R.,
Kouvelas E. D.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490230308
Subject(s) - adrenergic , adrenergic agent , neuroscience , computational biology , biology , medicine , receptor
In the present work [ 3 H]‐WB4101, [ 3 H]‐DHA, and [ 3 H]‐clenidine were used for the study of the localization of α1, α2, and βT adrenergic receptors in the chick brain. The highest concentration of [ 3 H]‐WB4101 was observed in the nucleus pretectalis, followed by the nucleus brachium conjuctivum descendens. The supercial layers of stratum griseum fibrosum superficiale, the nucleus mesencephalis lateralis pars dorsalis, and the locus coeruleus showed concentrations of [ 3 H]‐WB4101 binding higher than 300 fmoles/mg protein. Concentrations of [ 3 H]‐DHA binding higher than 300 fmoles/mg protein were observed in the paleostriatum, the external part of nucleus pretectalis, the nucleus isthmi parvocellularis, the nucleus mesencephalis lateralis pars dorsalis, the dorsal nucleus of oculomotor center, and the molecular layer of cerebellum. Locus coeruleus was the only area of chick brain which showed concentration of [ 3 H]‐clonidine binding higher than 300 fmoles/mg protein. With few exceptions, [ 3 H]‐clonidine binding was very low and in the telencephalon it was undetectable.