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Expression of nerve growth factor receptors by human peripheral blood mononuclear cells
Author(s) -
Morgan B.,
Thorpe L. W.,
Marchetti D.,
PerezPolo J. R.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490230106
Subject(s) - receptor , peripheral blood mononuclear cell , nerve growth factor , endocrinology , immune system , medicine , cell surface receptor , biology , cell , immunofluorescence , microbiology and biotechnology , chemistry , immunology , in vitro , antibody , biochemistry
In the rat, nerve growth factor (NGF) has been shown to affect immune reactivity by binding to cell surface receptors on a subpopulation of splenic mononuclear cells. This binding occurs in a specific and saturable fashion to what appear to be low‐affinity (type II) NGF receptors (NGFR). Immunofluorescence studies here showed that NGFR are also present on a proportion of human peripheral blood mononuclear cells (PBMC). Equilibrium binding studies demonstrated that the binding of NGF to its receptors on PBMC occurs with a single equilibrium binding constant (mean) of 2.11 × 10 −9 M. The number of receptors per cell was determined to be approximately 6.94 × 10 3 receptors/cell. These results would suggest a role for NGF in the regulation of immune function in man, as well as in animals.