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Age‐related modification of enzyme activities in synaptosomes isolated from rat cerebral cortex
Author(s) -
Curti D.,
Benzi G.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490220315
Subject(s) - phosphofructokinase , cytochrome c oxidase , enzyme , microcirculation , papaverine , cerebral cortex , dehydrogenase , citrate synthase , biochemistry , pyruvate dehydrogenase complex , chemistry , brain cortex , medicine , endocrinology , biology , glycolysis
In synaptosomes from rat cerebral cortex, the potential catalytic activity of some enzymes related to energy metabolism—namely, phosphofructokinase and citrate synthase—is not affected by aging. In contrast, the maximum velocity (V max ) of cytochrome oxidase and of pyruvate dehydrogenase decreases in aged rats. A marked increase is found in the V max of glucose 6‐phosphate dehydrogenase in aged rats and could be related to the availability of NADPH for antiperoxidative processes. Pretreatments of experimental animals with certain drugs were done to investigate the plasticity of enzyme proteins during aging. Papaverine, which acts on macrocirculation, is ineffective, but delta‐yohimbine acting on microcirculation and metabolism and almitrine acting on oxygen availability both could interfere with the potential activity of some enzymes. However, their influence differs with the age of the rats.