Evidence that the axolemmal mitogen for cultured schwann cells is a positively charged, heparan sulfate proteoglycan‐bound, heparin‐displaceable molecule | Zendy

Decoster M. A. | Zendy; Devries G. H. | Zendy

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Evidence that the axolemmal mitogen for cultured schwann cells is a positively charged, heparan sulfate proteoglycan‐bound, heparin‐displaceable molecule

Author(s) -

Decoster M. A.,

Devries G. H.

Publication year - 1989

Publication title -

journal of neuroscience research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.72

H-Index - 160

eISSN - 1097-4547

pISSN - 0360-4012

DOI - 10.1002/jnr.490220308

Subject(s) - axolemma , proteoglycan , heparin , mitogen activated protein kinase , sulfation , heparan sulfate , chemistry , microbiology and biotechnology , schwann cell , biochemistry , perlecan , biophysics , biology , signal transduction , endocrinology , myelin , extracellular matrix , central nervous system

Treatment of axolemma with pH 9 buffer results in a pellet enriched two‐fold in the mitogen for cultured Schwann cells. Heparitinase treatment releases 8% of the mitogen into solution, while heparin selectively solubilizes the mitogen, resulting in an extract which has a specific mitogenic activity approximately 2.5 times greater than the mitogenicity of the starting axolemma membrane. These data support a model in which the axolemmal mitogen is a positively charged molecule associated with negatively charged sulfated proteoglycans.

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