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Anti‐200 kDa neurofilament antibody cross‐reacts with microtubule‐associated protein‐2 (MAP‐2)
Author(s) -
Allende M. L.,
Krauss R. Y.,
Tremblay C.,
Alvarez J.,
Inestrosa N. C.
Publication year - 1989
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490220204
Subject(s) - microtubule , neurofilament , cytoskeleton , protein subunit , microtubule associated protein , organelle , epitope , tubulin , tau protein , microbiology and biotechnology , intermediate filament , biology , antibody , chemistry , biochemistry , immunohistochemistry , genetics , immunology , medicine , alzheimer's disease , cell , pathology , disease , gene
An antibody prepared against purified 200‐kDa neurofilament (NF) subunit was found to cross‐react with a high molecular weight polypeptide present in rat brain cytoskeletal extracts starting from early postnatal development. This polypeptide was found to be highly concentrated in microtubule preparations and was specifically recognized as the 280‐kDa microtubule‐associated protein‐2 (MAP‐2). Our results, besides suggesting the existence of common epitopes between the heavy NF subunit and MAP‐2, suggest the possibility of an interaction between two of the most important neuronal cytoskeletal organelles: NFs and microtubules.