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Expression of 48‐kilodalton intermediate filament‐associated protein in differentiating and in mature astrocytes in various regions of the central nervous system
Author(s) -
AbdElBasset E. M.,
Kalnins V. I.,
Fedoroff S.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490210215
Subject(s) - glial fibrillary acidic protein , cerebellum , white matter , central nervous system , astrocyte , brainstem , biology , spinal cord , intermediate filament , gfap stain , corpus callosum , neuroglia , grey matter , oligodendrocyte , pathology , neuroscience , immunohistochemistry , medicine , immunology , cytoskeleton , biochemistry , magnetic resonance imaging , myelin , cell , radiology
In this paper we present evidence that the 48‐kD intermediate filament‐associated protein (IFAP) is expressed relatively late in maturation of astrocytes, after they have acquired the glial fibrillary acidic protein (GFAP). In the astrocyes of white matter in the cerebellum the GFAP is detected at P3, whereas the 48‐kD IFAP is detected only at P11. In the periventricular region and the hippocampus the 48‐kD IFAP was detected at P6, long after the appearance of GFAP. In adult mice the 48‐kD IFAP was observed in GFAP‐positive astrocytes in the white matter of cerebellum, spinal cord, brainstem, and corpus callosum as well as in GFAP‐positive cells in the grey matter of cerebral cortex and spinal cord. The 48‐kD IFAP was not, however, detected in radial glia and their derivatives, in Bergmann glia or in Müller glia. Thus, not all the GFAP‐positive astroglia express the 48‐kD IFAP. Similarly, 48‐kD IFAP was not detected in cells which were GFAP‐negative.