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Gliogenesis in rat spinal cord: Evidence for origin of astrocytes and oligodendrocytes from radial precursors
Author(s) -
Hirano M.,
Goldman J. E.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490210208
Subject(s) - gliogenesis , oligodendrocyte , astrocyte , spinal cord , biology , neuroglia , microbiology and biotechnology , population , ependymal cell , forebrain , myelin , neuroscience , central nervous system , neural stem cell , stem cell , medicine , environmental health
We have examined glial cell lineages during rat spinal cord development by using a variety of antibodies that react with immature and mature glia. Radial glia in embryonic cord bound (1) A2B5, an antibody that reacts with a glial precursor cell population in optic nerve; (2) AbR24, which is directed against GD3 ganglioside and binds to immature neuroectodermal cells and to developing oligodendrocytes in forebrain and cerebellum; and (3) an antibody to the intermediate filament, vimentin. With time, two different populations emerged, both of which seemed to be derivatives of radial cells. One cell type expressed the astrocyte intermediate filament, GFAP, in addition to vimentin. GFAP‐containing cells eventually took on the forms of astrocytes in gray and white matter. The other type expressed carbonic anhydrase, an enzyme characteristic of oligodendrocytes and enriched in myelin. Carbonic anhydrase‐positive cells eventually developed into small cells with oligodendrocyte morphology. Our observations suggest a common lineage for astrocytes and oligodendrocytes from radial cells during spinal cord gliogenesis.