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Secretion of newly synthesized proteins into the extracellular fluid of the rabbit hippocampus
Author(s) -
Nyström B.,
Karlsson J.O.,
Hamberger A.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490210108
Subject(s) - secretion , extracellular , hippocampus , extracellular fluid , rabbit (cipher) , microbiology and biotechnology , chemistry , neuroscience , biophysics , endocrinology , biochemistry , biology , computer science , computer security
Extracellular proteins were collected continuously from the hippocampus of the rabbit by slow perfusion of a protein‐permeable thin tubing. After pulse labelling with radioactive amino acids via the tubing a rapid appearance of labelled proteins was seen in the extracellular fluid. The half‐life of the proteins in the extracellular space varied between 15 min and 2 h. Protein fractions were separated with gel electrophoresis or HPLC. The extracellular fraction differed distinctly from intracellular proteins with respect to the labelling pattern.