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Effects of acetylcholine and other agents on 32 P‐prelabeled phosphoinositides and phosphatidate in crude synaptosomal preparations
Author(s) -
White H. L.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490200117
Subject(s) - phosphatidic acid , oxotremorine , acetylcholine , chemistry , pirenzepine , carbachol , phosphatidate , muscarinic acetylcholine receptor , cholinergic , biochemistry , endocrinology , phospholipid , biology , receptor , membrane
Experimental conditions are described which permit effects of various agents on polyphosphoinositides and phosphatidic acid (PA) to be evaluated simultaneously in crude nerve‐ending preparations from rat brain. Acetylcholine (3‐100 μM) or carbachol (30‐1,000 μM) induced the hydrolysis of prelabeled polyphosphoinositides and, at the same time, stimulated the net label incorporated in phosphatidic acid. All muscarinic effects were blocked by atropine or pirenzepine. Nonmuscarinic agonists (glutamate, adenosine, norepinephrine) stimulated polyphosphoinositide hydrolysis in this preparation, but of these only norepinephrine affected phosphatidic acid turnover. A Potentiation of acetylcholine‐induced phosphoinositide turnover by KCI was observed, as well as an apparent selective inhibition of PIP2 hydrolysis by LiCl. Acetylcholinestimulated turnover of PA was not necessarily coupled to phosphoinositide hydrolysis.