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Fetal antigen retained by mature neurons and ependyma studied with a monoclonal antibody (6B9)
Author(s) -
Malhotra S. K.,
Predy R.,
Singh R.,
Gupta B. L.,
Bhatnagar R.,
Leeuw K.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490190406
Subject(s) - antigen , monoclonal antibody , biology , immunoelectron microscopy , ependyma , microbiology and biotechnology , pathology , ependymal cell , immunofluorescence , antibody , immunocytochemistry , immunohistochemistry , immunology , central nervous system , endocrinology , medicine
A mouse monoclonal antibody (MAb 6B9, isotype IgM) was raised against autopsy tissue sample from the central nervous system (CNS) of multiple sclerosis (MS) patients. By immunofluorescence microscopy, MAb 6B9 intensely stain most or all cell in fetal rats. However, MAb 6B9 differentially stains various cell types in adult rats. Neurons, ependymal cells, and adrenal chromaffin cells are stained intensely, whereas astrocytes and oligodendrocytes are not stained. The 6B9‐reactive antigen (6B9 antigen) is senstive to peodic acid, but insentive to treatment with protease, RNase, or hyaluronidase. Result form immunfluo‐‐microscopyon something section and cultured neuroblastoma cells indicated that 6B9 antigen is intracellular. This is supported by immunoelectron microscopy, where lableing for 6B9 antigen appear in the cytoplasm distinct from any identifiable organelle. Further studies on 6B9 antigen should reveal its chemical nature as well as the significance of developmental changes in its distribution.