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Enhanced expression of a protein antigen (JI‐31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord
Author(s) -
Predy R.,
Malhotra S. K.,
Das G. D.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490190403
Subject(s) - antigen , glial fibrillary acidic protein , astrocyte , spinal cord , immunofluorescence , pathology , microbiology and biotechnology , biology , monoclonal antibody , staining , immunohistochemistry , antibody , immunology , chemistry , medicine , central nervous system , endocrinology , neuroscience
A mouse monoclonal antibody IMAb JI‐31, isotype IgG 2b was raised against an autopsy sample of cerebral white matter from a multiple sclerosis (MS) patient. MAb JI‐31 recognizes a protein (JI‐31 antigen) in human brain which has a molecular weight of approximately 30,000 daltons (30 kD) as determined by immunoprecipitaion followed by SDS‐gel electrophoresis (reducing conditions) and autoradiography (Singh et al.: Biosci Rep 6:73–79,1986). By immunofluorescence micoroscopy,. Mab JI‐31 stains glial fibrillary acidic protein (GFAP)‐positive cells, namely astrocytes from GFAP (Predy et al.: Biosci Rep 7:491–502,1987). In this paper we report we report that greatly enhanced staining for JI‐31 antigen is exhibited by reactive astrocytes which arise following CNS injury. (Laceration‐tape surgical lesion of the rat spinal cord served as the expermental modle.) Enhanced expression of JI‐31 antigen reveals some new aspect of the astrocyte response to injury, involving transformation to the reactive state. Consequently, MAb Jl‐31 may be a useful marker for studies or reactive astrocytes.