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A chemically defined medium for the culture of mature oligodendrocytes
Author(s) -
De Los Monteros A. Espinosa,
Roussel G.,
Neskovic N. M.,
Nussbaum J. L.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490190205
Subject(s) - chemically defined medium , chemistry , microbiology and biotechnology , biology , biochemistry , in vitro
A new chemically defined medium consisting of equal parts of Dulbecco modified Eagle's and Ham's F‐12 media supplemented with insulin, sodium selenite, putrescine, and D + galactose, which allows the longterm survival of mature oligodendrocyte pure cultures, is described. Immunohistochemical staining has shown that over 90% of the cells become positive for myelin proteins shortly following subculture. Contaminating astrocytes (2%) do not survive in this medium. Biochemical data have indicated that these purified oligodendrocytes express 2′ 3′‐cyclic nucleotide 3′‐phosphohydrolase and UDP‐galactose ceramide galactosyltransferase activities. Electoron microscopical examination revealed that the oligodendrocytes were mostly of medium‐dark type and appeared to be identical to cells cultured in serum‐containing medium. The ability to maintain pure oligodendrocyte cultures in such a defined medium will allow investigations concerning exogenous and endogenous factors involved in oligodendrocyte metabolism.