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Occurrence of phospholipase A 1 –A 2 and lysophosphatidylcholine acyltransferase activities in axolemma‐enriched fractions of brain stem, optic pathway, and cranio‐spinal nerves of the rabbit
Author(s) -
Alberghina M.,
Buonacera P.,
Agodi A.,
Stella A. M. Giuffrida
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490190111
Subject(s) - axolemma , acetylcholinesterase , central nervous system , biochemistry , phospholipase c , biology , lysophosphatidylcholine , phospholipase , peripheral nervous system , enzyme , chemistry , anatomy , endocrinology , myelin , phosphatidylcholine , membrane , phospholipid
An axolemma‐enriched fraction was isolated and characterized from homogenates of brain stem, pooled optic nerve and tract, and sciatic and hypoglossal nerves of adult rabbits. In these fractions, the phospholipase A 1 and A 2 , as well as the activity of acylCoA:2 ‐ acyl ‐ sn ‐ glycero ‐ 3 ‐ phosphorylcholine acetyl transferase, using 1‐acyl‐ and 2‐acyl‐GPC as acyl acceptors, were studied. The activity of the four enzymes was clearly detecable in the central nervous system (CNS) and peripheral nervous system (PNS) axolemmatic perparations, as well as in other subcellular fractions examined. The axolemma fracions, in which acetylcholinesterase displayed the highest activities, were particularlyenriched in the acylatin reaction enzymes. These latter showed specific activities about twofold higher compared with those of the homogenates and significant correlation with acetylcholinesterase. The noticeable presence of these enzymeactivities in both CNS and PNS axolemma suggests that a deacylation–reacylation system for phospholipids may be operative in this membrane.