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Developmental effect of dimethyl sulfoxide on hypothalamo‐neurohypophysial neurons in vitro
Author(s) -
Schilling K. L.,
Pilgrim C.
Publication year - 1988
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490190105
Subject(s) - immunocytochemistry , dimethyl sulfoxide , neurophysins , enolase , cell culture , in vitro , neuron , biology , staining , biochemistry , chemistry , microbiology and biotechnology , endocrinology , immunohistochemistry , immunology , neuroscience , hormone , genetics , organic chemistry
Primary dissociated cultures were established from diencephalic tissue of 14‐day‐old fetal rats. Neurons exhibiting immunocytochemical staining for neurophsin appeared in these cultures after 6 days of cultivation. Addition of dimethyl sulfoxide (DMSO) to the culture medium resulted in a slight decrease in total neuronal cell mass as assessed by immunocytochemistry and radio‐immunometric quantitation of neuron‐specific enolase. In contrast, in DMSO‐treated cultures the number of neurophysin‐immunoreactive neurons was more than doubled as compared to control cultures. [ 3 H] Thymidine labeling and autoradiography in conjuction with immunocytochemistry for neurophysin showed that this was not due to a mitogenic effect of DMSO on precursor cells. Time‐course analysis of the action of DMSO revealed a 6‐day time lag between the initiation of treatment and the appearance of increased numbers of neurophysin‐immunoreactive cells. These findings suggest that DMSO, which has previously been on malignant transformed cells, may also modulate cellular processes that control differentiation in specific types of neurons in primary culture.