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Changes in the pattern of expression of pp 60c‐ src in cerebellar mutants of mice
Author(s) -
Brugge J. S.,
Lustig A.,
Messer A.
Publication year - 1987
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490180405
Subject(s) - rous sarcoma virus , proto oncogene tyrosine protein kinase src , mutant , biology , microbiology and biotechnology , phosphorylation , genetics , gene
Cultures of neurons from rat embroyos have been show previously to express high levels of a unique from of pp 60c‐ src [Brugge et al (1985): Nature 316:524‐526], the cellular homologue of the transforming protien of Rous sarcoma virus. This altered form of pp 60c‐ src (+), designated pp 60c‐ src (+), displays a retarded electroretic mobility due to a structural alteration within the aminoterminal region of the molecule [Brugge et al, 1985]. In order to investigate the distribution and possible role of pp 60c‐ src (+) in intact brain, we have examined the expression of pp 60c‐ src in extracts from developing cerebella from wild‐type mice and mutant mice that display progressive degeneration of specific classes of cerebellar neurons. The loss of pp 60c‐ src (+) generally correlated with the loss of granule cells and Purkinje cells from the cerebella of mice carrying the staggerer (sg/sg) and Lurcher (Lc/+) mutation, with the most pronounced changes observed in cerebella from the more severely affected sg/sg mice. The experssion of pp 60c‐ src (+) in weaver wv/wv mice is qualitatively and quantitatively quite different. From the earliest time points, there was a significant reduction in the levels of pp 60c‐ src (+), with no further loss of this form during the period of maximal neuronal differentiation. This suggests an maximal neuronal differentiation. This suggests an early, predegenerative absence of pp 60c‐ src (+) in this mutant strian, which is defective in granule‐cell migration.