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Nerve growth factor immunoreactivity in mouse kidney: An immunoelectron microscopic study
Author(s) -
Barajas L.,
Salido E. C.,
Laborde N. P.,
Fisher D. A.
Publication year - 1987
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490180306
Subject(s) - immunoelectron microscopy , immunoperoxidase , immunostaining , golgi apparatus , endoplasmic reticulum , nerve growth factor , ultrastructure , microbiology and biotechnology , pathology , electron microscope , biology , immunohistochemistry , chemistry , medicine , antibody , immunology , monoclonal antibody , receptor , biochemistry , physics , optics
We have recently reported the immunolocalization of nerve growth factor (NGF) in mouse kidney by light microscopy. In the present study, we have investigated the ultrastructural localization of NGF by the preembedding immunoperoxidase method for electron microscopy. NGF immunoreactivity was present in the connecting tubule cells of the distal nephron. These cells showed immunostaining associated with the Golgi complex, vesicles, rough endoplasmic reticulum (RER), and polyribosomes. The intercalated cells, in contrast, lacked immunoreactivity.