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Arachidonic acid inhibits uptake of glutamate and glutamine but not of GABA in cultured cerebellar granule cells
Author(s) -
Yu A. C. H.,
Chan P. H.,
Fishman R. A.
Publication year - 1987
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490170414
Subject(s) - glutamate receptor , granule (geology) , glutamine , cerebellum , arachidonic acid , granule cell , astrocyte , gamma aminobutyric acid , glutamic acid , biology , chemistry , microbiology and biotechnology , neuroscience , biochemistry , central nervous system , amino acid , dentate gyrus , receptor , enzyme , paleontology
Abstract The effects of arachidonic acid (20:4) on the uptake of glutamate were studied in primary cultures of cerebellar granule cells and were compared to cortical neurons and astrocytes. At a dose of 0.005 mM, the glutamate uptake was significantly inhibited in cerebellar granule cells. This inhibition was dose and time dependent. The uptake of glutamate was equally sensitive to 20:4 in primary cell cultures of cortical neurons, whereas the uptake in astrocytes was much less sensitive to 20:4. Glutamine uptake was inhibited by 20:4 in cultured cerebellar granule cells and cerebral cortical astrocytes but was not affected in cerebral cortical neurons. Furthermore, the uptake of gammaaminobutyric acid was not affected by 20:4 in cerebellar granule cells.