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Effect of ascorbic acid on the synaptosomal uptake of [ 3 H]MPP + , [ 3 H]Dopamine, and [ 14 C]GABA
Author(s) -
Sershen H.,
Debler E. A.,
Lajtha A.
Publication year - 1987
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490170315
Subject(s) - ascorbic acid , dopamine , chemistry , radiochemistry , endocrinology , medicine , food science
The effects of ascorbic acid on the synaptosomal uptake of [ 3 H]MPP + , [ 3 H]dopamine, and [ 14 C]GABA were examined in attempts to understand the mechanism of ascorbic acid attenuation of MPTP neurotoxicity. [ 3 H]Dopamine uptake was increased at lower levels (0.01 and 0.1 mM) and decreased at higher levels (10 mM) of ascorbic acid. Ascorbic acid inhibited [ 3 H]MPP + uptake (IC 50 = 0.1 mM) and [ 14 C]GABA uptake (IC 50 = 10 mM). Washout of ascorbic acid restored uptake of [ 3 H]dopamine and [ 3 H]MPP + , suggesting that ascorbic‐acid‐induced lipid peroxidation was not involved in the effect on uptake. In addition to the possible involvement of antioxidant mechanisms in the in vivo attenuation of the neurotoxicity of MPTP by ascorbic acid, the present results indicate a direct effect of ascorbic acid in inhibiting the uptake of MPP + into dopaminergic nerve terminals.