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The survival of adult mouse sensory neurons in vitro is enhanced by natural and synthetic substrata, particularly fibronectin
Author(s) -
Smith R. A.,
Orr D. J.
Publication year - 1987
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490170310
Subject(s) - fibronectin , cytosine , biology , polylysine , in vitro , population , neuron , motility , dorsum , microbiology and biotechnology , cell , biochemistry , anatomy , neuroscience , gene , medicine , environmental health
Primary cultures derived from adult mouse dorsal root ganglia have been maintained in the presence or absence of 5 × 10 −6 M cytosine arabinoside for periods of up to 4 weeks. In cultures in which cytosine arabinoside is present, the non‐neuronal cell population is effectively reduced. When uncoated plastic substrata are used there is also a concurrent decrease in the number of neurons if the medium is supplemented with cytosine arabinoside. The effects on neuron survival of substrata coated with fibronectin, polyornithine, polylysine, and exudates prepared from mouse liver cells were studied. It was shown that neuronal densities similar to those with uninhibited media may be retained in the presence of cytosine arabinoside if fibronectin‐coated substrata are prepared. With the other coating agents neuronal survival was also enhanced but to a lesser extent. The study offers a means therefore of producing purer cultures of dorsal root ganglia neurons than has previously been possible from adult mammalian sources.