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Localization of proopiomelanocortin mRNA in functional subsets of neurons defined by their axonal projections
Author(s) -
Wilcox J.N.,
Roberts J.L.,
Chronwall B.M.,
Bishop J.F.,
O'Donohue T.
Publication year - 1986
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490160109
Subject(s) - proopiomelanocortin , neuroscience , messenger rna , biology , gene , genetics , hypothalamus
In situ cDNA:mRNA hybridization is a technique that has been developed for the visualization of cDNA:mRNA hybrids in individual cells. To use this technique to answer questions of regulation in heterogeneous populations of cells in the brain, it must be combined with other procedures allowing for the identification of functional subgroups of neurons. We report here a procedure by which in situ cDNA:mRNA hybridization may be combined with retrograde axonal tracing using the fluorescent tracer fast blue. Using this technique, it now becomes possible to measure mRNA regulation in functional subsets of cells defined by their axonal projections.