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Hypotaurine uptake by the retina
Author(s) -
PasantesMorales H.,
Morán J.,
Fellman J. H.
Publication year - 1986
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490150110
Subject(s) - hypotaurine , taurine , retina , biochemistry , biology , glycine , biophysics , chemistry , amino acid , neuroscience
A study of the transport of 3 H‐hypotaurine by rat retina has been carried out. The process is markedly sodium and temperature dependent. Kinetic analysis of the data revealed two values for the affinity constant (K) of 4.91 μM and 1,071.3 μM. Some related and unrelated amino acids were examined as competitive inhibitors of 3 H‐hypotaurine transport. Only GABA and β‐alanine exhibited appreciable inhibitory activity. Taurine, glycine, and guanidine ethane sulfonate were not inhibitors. 3 H‐hypotaurine uptake was similarly distributed in the retinal subcellular fractions P 1 and P 2 . The active uptake of 3 H‐Hypotaurine by retinas of the rat and other species including chick and frog was studied and compared to cerebral cortex slices. The chick retina was similar to the rat in its capacity to transport hypotaurine whereas frog exhibited fourfold less activity. Slices of cerebral cortex from rat and chick exhibited hypotaurine uptake but again the magnitude of the transport was three to fourfold less than in the comparative retinas.