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Growth patterns of glial cells dissociated from newbron and aged mouse brain with cell passage
Author(s) -
Vernadakis A.,
Davies D.,
Sakellaridis N.,
Mangoura D.
Publication year - 1986
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490150108
Subject(s) - glial fibrillary acidic protein , glutamine synthetase , biology , senescence , multinucleate , cell , astrocyte , glutamine , neuroglia , cell culture , microbiology and biotechnology , microglia , gfap stain , biochemistry , central nervous system , immunology , endocrinology , genetics , immunohistochemistry , amino acid , inflammation
Abstract Glial cell cultures derived from newbron and aged (18‐month‐old) mouse cerebral hemispheres and maintained up to cell passage 11 were characterized immunocytochemically by using glial fibrillary acidic protein (GFA), and biochemically by using glutamine synthetase (GS), for astrocytes, and 2′,3′ cyclic nucleotide 3′ phosphohydrolase (CNP) for oligodendrocytes. We report here the changes occurring during passages 5–1. GS and CNP activities did not significantly changes with cell passage in cultures from newbron mouse. In cultures derived from aged mouse, CNP activity did not change signigicantly whereas GS activity increased severalfod. A characteristic finging in higher cell passages (passage #7) was the loss of GFA‐positive stained cells and the appearance of multinucleated cells. We interpret these changes in culture to represent possible signs of cellular senescence.