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Synthesis of a myelin‐like membrane by oligodendrocytes in culture
Author(s) -
Rome L. H.,
Bullock P. N.,
Chiappelli F.,
Cardwell M.,
Adinolfi A. M.,
Swanson D.
Publication year - 1986
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490150106
Subject(s) - myelin , oligodendrocyte , transferrin , in vivo , biology , microbiology and biotechnology , central nervous system , lysis , myelin basic protein , membrane , immunocytochemistry , neuroglia , in vitro , chemistry , biochemistry , biophysics , neuroscience , endocrinology , genetics
Abstract We have prepared highly purified cultures of rat oligodendrocytes by a modification of the procedure of McCarthy and de Vellis [1980]. By utilizing a substratum derived from lysed glia and a calf serum‐containing medium with a high concentration of transferrin, the oligodendrocyte cultures display a high degree of purity, the ability to survive several months of culture, and a striking ability to produce a myelin‐like membrane. We have examined the production of this myelin‐like membrane using immunocytochemical and biochemical probes as well as an extensive morphological examination at the electron microscopic level. The membrane appears to be produced in a similar developmental pattern to that observed in vivo and it has the structural characteristics of loosely packed central nervous system myelin.