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Effects of tunicamycin on NGF binding and neurite outgrowth in PC12 cells
Author(s) -
Baribault T. J.,
Neet Kenneth E.
Publication year - 1985
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490140105
Subject(s) - tunicamycin , neurite , nerve growth factor , receptor , microbiology and biotechnology , glycosylation , chemistry , biology , endocrinology , biochemistry , in vitro , endoplasmic reticulum , unfolded protein response
The effects of inhibition of glycosylation on nerve growth factor (NGF) binding and neurite outgrowth response of PC12 cells have been examined. Exposure to tunicamycin (1–10 μg/ml) for 24–36 hr eliminates the rapidly dissociating component of NGF binding and decreases the decreases the proportion of PC12 cells capable of elaborating neurits in a dose‐dependent manner. These decreased cellular responses are probably due to an underglycosylation of the NGF receptor, since the effects of tunicamycin are correlated with a decrease in 3 H‐fucose incorporation rather than a general decline in cellular metabolism as measured by viability and protein syntheses. These results suggest that carbohydrate side chains are important for the function and/or orientation of the NGF receptor in PC12 cells and that the rapidly dissociating component of NGF binding may be associated with a minimum concentration of functional receptors per cell required for the full biologic response.