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Immunohistochemical localization of myelin basic protein in the developing optic system of trout
Author(s) -
Jeserich G.,
Jacque C.
Publication year - 1985
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490130408
Subject(s) - tectum , biology , myelin , immunohistochemistry , anatomy , myelin basic protein , antiserum , immunofluorescence , optic nerve , microbiology and biotechnology , central nervous system , neuroscience , midbrain , antigen , immunology , antibody
Immunofluorescent localization of basic protein (BP) in the CNS of trout was performed using a heterologous antiserum raised against human BP. Bright specific fluorescence was confined to the myelin sheath of axons in most regions of the brain, whereas neuronal pericarya and dendrites as well as glial cells were entirely negative. In the tectum immunofluorescence was characteristically distributed in two rows of horizontally aligned patches, most evidently reflecting the orderly array of myelinated fiber bundles in the stratum opticum and stratum album. During development of the retino‐tectal pathway, the appearance of BP followed a distinct rostro‐caudal gradient firstly reaching the anterior ventral border of the tectum by larval stage 34. Furthermore, on cross sections through the optic nerve a specifically organized pattern of myelin formation was revealed by immunohistochemistry, which strikingly coincided with the pattern of fiber outgrowth.