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Tryptophan fluorescence properties of cholera toxin upon interacting with ganglioside GD 1b
Author(s) -
Mestrallet M. G.,
Bennun F. R.,
Maggio B.,
Cumar F. A.
Publication year - 1984
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490120220
Subject(s) - ganglioside , tryptophan , cholera toxin , chemistry , toxin , fluorescence , quenching (fluorescence) , iodide , biophysics , biochemistry , biology , microbiology and biotechnology , inorganic chemistry , amino acid , physics , quantum mechanics
A blue shift of the tryptophan fluorescence emission spectra of cholera toxin or the B protomer is induced by disialoganglioside GD 1b with a capacity similar to that of monosialoganglioside GM 1 . Both gangliosides were also capable of decreasing or reversing the fluorescence quenching by iodide ion of the toxin. The quenching constants (K sv ) for the toxin fluorescence in absence of gangliosides was 2.8 M −1 ; in presence of GM 1 or GD 1b , K sv was 0.8 M −1 and 0.7 M −1 , respectively. Gangliosides GD 1a and GT 1b were unable to decrease the quenching effect. The results suggest that GD 1b induces a perturbation in the tryptophan environment of the toxin molecule similar to that induced by GM 1 .