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Inhibition of the chicken retinal UDP‐GalNAc:GM3, N‐acetylgalactosaminyltransferase by blood serum and by pineal gland extracts
Author(s) -
Quiroga S.,
Caputto B. L.,
Caputto R.
Publication year - 1984
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490120214
Subject(s) - pineal gland , retinal , in vivo , inhibitory postsynaptic potential , biology , in vitro , endocrinology , retina , medicine , chemistry , biochemistry , melatonin , neuroscience , microbiology and biotechnology
The effects of the pineal gland extract and blood serum on the activity of the UDP‐GalNAc:GM3, N‐acetylgalactosaminyltransferase (GM3:GalNAc‐T) from chicken retina were studied. Both preparations have inhibitory capability on the enzyme activity. Two types of inhibitory capabilities were found: one is heat labile and decomposes UDP‐GalNAc and another is heat stable. When the pineal gland extracts were prepared from light‐exposed chickens, the inhibitory capability increased with respect to the extracts from dark‐maintained animals; vice versa, blood serum from dark‐maintained animals had higher heat‐labile and heat‐stable capabilities than that from light‐exposed chickens. In in vitro experiments, no difference was found in the inhibitory capability of blood serum extracts from pinealectomized animals compared to control animals. In vivo labeling experiments with pinealectomized animals in either light or dark showed similar differences in the labeling of the optic tectum gangliosides as the normal animals.