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Cytosolic gangliosides of rat brain: Their fractionation into protein‐bound complexes of different ganglioside compositions
Author(s) -
Sonnino S.,
Ghidoni R.,
Fiorilli A.,
Venerando B.,
Tettamanti G.
Publication year - 1984
Publication title -
journal of neuroscience research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.72
H-Index - 160
eISSN - 1097-4547
pISSN - 0360-4012
DOI - 10.1002/jnr.490120207
Subject(s) - ganglioside , cytosol , sialic acid , fractionation , biochemistry , chemistry , column chromatography , chromatography , enzyme
The sialic acid moiety of rat brain cytosolic gangliosides was radiolabeled by intracranial injection of N‐( 3 H)acetylmannosamine. Upon ammonium sulphate fractionation, Sepharose 6B gel filtration, and hydroxylapatite‐cellulose chromatography, ganglioside‐bound radioactivity of brain cytosolic extract followed the behavior of protein and not that of purified gangliosides. This indicates that cytosolic gangliosides occur as ganglioside‐protein complexes. By application of hydroxylapatite‐cellulose column chromatography, fractions were obtained having different ganglioside composition. In particular, one fraction contained GM1, one GD1a, and one GT1b with a ganglioside homogeneity better than 95% in each fraction. This indicates the occurrence in brain cytosol of a GM1‐protein complex, a GD1a‐protein complex, and a GT1b‐protein complex.